Dr Jan Dolfing
Senior Research Associate
Telephone: +44 (0) 191 222 8352
Background
Joined the School of Civil Engineering and Geosciences at Newcastle in 2006 as part of ECOSERV, an EU-funded excellence team integrating molecular measurement methods, ecological theories, and mathematics to determine fundamental rules that govern the behaviour of biological treatment communities. His research interests focus on the thermodynamic logic behind microbial interactions in anaerobic ecosystems (with applications in anaerobic wastewater treatment, methanogenic bioreactors and microbial fuel cells).
Qualifications
PhD Microbiology (Wageningen University, 1987) MEng Environmental Engineering (Wageningen University, 1980)
Professional Experience
Engineer with over 25 years experience in environmental applications of chemistry and biology. Well known for his pioneering work on reductive dechlorination. Authored numerous reports on anaerobic digestion, emission of greenhouse gases, and immobilisation of heavy metals. Ongoing projects include: Reductive dechlorination of chlordecone, a legacy compound used for pest control in the French West Indies.
Previous Positions
Post-doc at Michigan State University, USA Post-doc at EAWAG/ETH in Switzerland Post-doc at the University of Groningen, the Netherlands Senior scientist at Alterra, Wageningen, the Netherlands
Memberships
Dutch Society for Microbiology International Society for Microbial Ecology American Society for Microbiology
Research interests
My main interest is in the applicability and application of thermodynamic logic to microbial processes and interactions in methanogenic ecosystems. Simple high school chemistry goes a long way to explaining and indeed understanding the hierarchy of microbial niches in the environment and the processes that these organisms catalyse. This approach resulted for example in the formulation of the hypothesis that microorganisms can obtain energy for growth from reductive dechlorination of halogenated organic compounds, a hypothesis that was subsequently experimentally validated. Other examples are the rationalization of why syntrophs in methanogenic ecosystems catalyse incomplete oxidation of organic compounds to acetate and H2 , and the “window of opportunity” concept for the methanogenic biodegradation of crude oil. Current ideas revolve about a more in depth understanding of the interactions and ecophysiology of the organisms involved in interspecies hydrogen transfer. Ultimately this modelling and experimentation based understanding will allow us to better engineer or engineer better anaerobic wastewater treatment systems.
Postgraduate supervision
Liz Heidrich working on “hydrogen production from wastewater using microbial electrolysis cells”
Evangelos Petropoulos who is “Investigating the true limits to low temperature anaerobic treatment of domestic wastewater”.
Esteem Indicators
Reviewer for NERC and BBSRC.
Member of the editorial board of Applied and Environmental Microbiology and of Microbial Ecology (past).
Invited speaker, all expenses paid:
Granulation in UASB reactors @ Anaerobic Digestion in Tropical Countries, Sao Paulo, 1985;
Hydrogen cycling in dechlorinating ecosystems @ ACS, Washington 2000;
Reductive dechlorination of Chlordecone @ Chlordecone in the French West Indies, 2010.
Funded projects
What is the true temperature limit for the anaerobic treatment of domestic wastewater? EPSRC 2009-2012, £654,848
Energy-use minimization in residuals management in the personal care product industry EU FP7 2008-2011, £198,839
Can understanding the emergent behaviour of mixed culture systems aid wastewater system design and operation? EPSRC 2007-2009, £151,494
Undergraduate Teaching
none
Postgraduate Teaching
none
Westerholm M, Dolfing J, Sherry A, Gray ND, Head IM, Schnürer A. Syntrophic acetate-oxidizing microbial communities in biogas processes .
Environmental Microbiology Reports 2011. In Press.
Heidrich ES, Curtis TP, Dolfing J. Determination of the internal chemical energy of wastewater .
Environmental Science & Technology 2011, 45 (2), 827-832.
Xu AP, Dolfing J, Curtis TP, Montague G, Martin E. Maintenance affects the stability of a two-tiered microbial 'food chain'? .
Journal of Theoretical Biology 2011, 276 (1), 35-41.
Kool DM, Dolfing J, Wrage N, Van Groenigen JW. Nitrifier denitrification as a distinct and significant source of nitrous oxide from soil .
Soil Biology & Biochemistry 2011,43 1 174-178.
Gray ND, Sherry A, Hubert C, Dolfing J, Head IM. Methanogenic degradation of petroleum hydrocarbons in subsurface environments: remediation, heavy oil formation, and energy recovery .
Advances in Applied Microbiology 2010, 72 , 137-161.
Dolfing J, Xu AP, Head IM. Anomalous energy yields in thermodynamic calculations: importance of accounting for pH-dependent organic acid speciation .
ISME Journal 2010. , 4 (4), 463-464.
Knapp CW, Dolfing J, Ehlert PAI, Graham DW. Evidence of increasing antibiotic resistance gene abundances in archived soils since 1940 .
Environmental Science & Technology 2010,44 2 580-587.
Dolfing J, Xu A, Gray ND, Larter SR, Head IM. The thermodynamic landscape of methanogenic PAH degradation .
Microbial Biotechnology 2009, 2 (5), 566-574.
Dolfing J, Larter SR, Head IM. Thermodynamic constraints on methanogenic crude oil biodegradation .
The ISME Journal 2008, 2 (4), 442-452.
Dolfing J, Van Eekert M, Seech A, Vogan J, Mueller J. In situ chemical reduction (ISCR) technologies: significance of low Eh reactions .
Soil & Sediment Contamination 2008, 17 (1), 63-74.
Dolfing J. Thermodynamic considerations for dehalogenation .
In: Häggblom MM; Bossert ID, ed. Dehalogenation: Microbial processes and environmental applications . Boston: Kluwer Academic Publishers, 2003, pp. 89-113.
Tzeneva VA, Salles JF, Naumova N, de Vos WM, Kuikman PJ, Dolfing J, Smidt H. Effect of soil sample preservation, compared to the effect of other environmental variables, on bacterial and eukaryotic diversity .
Research in Microbiology 2009, 160 (2), 89-98.
Sherry A, Gray ND, Aitken CM, Dolfing J. Microbial oil degradation under methanogenic conditions .
In: Timmis, K.N., ed. Handbook of Hydrocarbon and Lipid Microbiology .Heidelberg, Germany:Springer,2010, pp. 3906-3917.
Velthof GL, Hoving IE, Dolfing J, Smit A, Kuikman PJ, Oenema O. Method and timing of grassland renovation affects herbage yield, nitrate leaching, and nitrous oxide emission in intensively managed grasslands .
Nutrient Cycling in Agroecosystems 2010, 86 (3), 401-412.
Parsons JR, Sáez M, Dolfing J, de Voogt P. Biodegradation of perfluorinated compounds .
Reviews of Environmental Contamination and Toxicology 2008, 196 , 53-71.
Dolfing J, Jiang B, Henstra AM, Stams AJM, Plugge CM. Syntrophic growth on formate: a new microbial niche in anoxic environments .
Applied and Environmental Microbiology 2008, 74 (19), 6126-6131.
van der Salm C, Dolfing J, Heinen M, Velthof GL. Estimation of nitrogen losses via denitrification from a heavy clay soil under grass .
Agriculture, Ecosystems & Environment 2007, 119 (3-4), 311-319.
Kool DM, Wrage N, Oenema O, Dolfing J, Van Groenigen JW. Oxygen exchange between (de)nitrification intermediates and H2O and its implications for source determination of NO3- and N2O: a review .
Rapid Communications in Mass Spectrometry 2007, 21 (22), 3569-3578.
Stams AJM, de Bok FAM, Plugge CM, van Eekert MHA, Dolfing J, Schraa G. Exocellular electron transfer in anaerobic microbial communities .
Environmental Microbiology 2006, 8 (3), 371-382.
Oenema O, Wrage N, Velthof GL, van Groenigen JW, Dolfing J, Kuikman PJ. Trends in global nitrous oxide emissions from animal production systems .
Nutrient Cycling in Agroecosystems 2005, 72 (1), 51-65.
Dolfing J, Vos A, Bloem J, Ehlert PAI, Naumova NB, Kuikman PJ. Microbial diversity in archived soils .
Science 2004. , 306 (5697), 813-813.
Koopmans GF, Chardon WJ, Ehlert PAI, Dolfing J, Suurs RAA, Oenema O, van Riemsdijk WH. Phosphorus availability for plant uptake in a phosphorus-enriched noncalcareous sandy soil .
Journal of Environmental Quality 2004, 33 (3), 965-975.
Mogensen AS, Dolfing J, Haagensen F, Ahring BK. Potential for anaerobic conversion of xenobiotics .
In: Birgitte K.Ahring, ed. Biomethanation II . Berlin: Springer-Verlag, 2003, pp. 69-134.
Koopmans GF, Chardon WJ, Dolfing J, Oenema O, van der Meer P, van Riemsdijk WH. Wet chemical and phosphorus-31 nuclear magnetic resonance analysis of phosphorus speciation in a sandy soil receiving long-term fertilizer or animal manure applications .
Journal of Environmental Quality 2003, 32 (1), 287-295.
Dolfing J. Nitrous oxide formation in the Colne estuary in England: the central role of nitrite .
Applied and Environmental Microbiology 2002. , 68 (10), 5202-5202.
Koopmans GF, McDowell RW, Chardon WJ, Oenema O, Dolfing J. Soil phosphorus quantity-intensity relationships to predict increased soil phosphorus loss to overland and subsurface flow .
Chemosphere 2002, 48 (7), 679-687.
Olivas Y, Dolfing J, Smith GB. The influence of redox potential on the degradation of halogenated methanes .
Environmental Toxicology and Chemistry 2002, 21 (3), 493-499.
Oste LA, Dolfing J, Ma WC, Lexmond TM. Cadmium uptake by earthworms as related to the availability in the soil and the intestine .
Environmental Toxicology and Chemistry 2001, 20 (8), 1785-1791.
Oste LA, Dolfing J, Ma WC, Lexmond TM. Effect of beringite on cadmium and zinc uptake by plants and earthworms: More than a liming effect? .
Environmental Toxicology and Chemistry 2001, 20 (6), 1339-1345.
Koopmans GF, van der Zeeuw ME, Chardon WJ, Dolfing J. Selective extraction of labile phosphorus using dialysis membrane tubes filled with hydrous iron hydroxide .
Soil Science 2001, 166 (7), 475-483.
Dolfing J. The microbial logic behind the prevalence of incomplete oxidation of organic compounds by acetogenic bacteria in methanogenic environments .
Microbial Ecology 2001, 41 (2), 83-89.
Griffiths BS, Ritz K, Bardgett RD, Cook R, Christensen S, Ekelund F, Sørensen SJ, Bååth E, Bloem J, de Ruiter PC, Dolfing J, Nicolardot B. Ecosystem response of pasture soil communities to fumigation-induced microbial diversity reductions: an examination of the biodiversity-ecosystem function relationship .
Oikos 2000, 90 (2), 279-294.
Dolfing J. Energetics of anaerobic degradation pathways of chlorinated aliphatic compounds .
Microbial Ecology 2000, 40 (1), 2-7.
Dolfing J, Chardon WJ, Japenga J. Association between colloidal iron, aluminum, phosphorus, and humic acids .
Soil Science 1999, 164 (3), 171-179.
Dolfing J, Bril J. Bioaccumulation factors as a function of soil characteristics .
Environmental Toxicology and Chemistry 1999. , 18 (12), 2665-2666.
Zevenhuizen LPTM, Dolfing J, Eshuis EJ, Scholten-Koerselman IJ. Inhibitory effects of copper on bacteria related to the free ion concentration .
Microbial Ecology 1979, 5 (2), 139-146.
Dolfing J. Comment on "Competition for hydrogen within a chlorinated solvent dehalogenating anaerobic mixed culture" .
Environmental Science & Technology 1999. , 33 (12), 2127-2127.
Dolfing J. Comment on "Degradation of 1,1,2,2-tetrachloroethane in a freshwater tidal wetland: Field and laboratory evidence" .
Environmental Science & Technology 1999. , 33 (15), 2680-2680.
Dolfing J. Comment on "Methane as a product of chloroethene biodegradation under methanogenic conditions" .
Environmental Science & Technology 1999, 33 (13), 2302-2303.
Römkens P, Hoenderboom G, Dolfing J. Copper solution geochemistry in arable soils: Field observations and model application .
Journal of Environmental Quality 1999, 28 (3), 776-783.
Dolfing J, Scheltens SD. Facilitated transport in European soils from the Euro-soil project .
Environmental Toxicology and Chemistry 1999, 18 (7), 1417-1420.
Dolfing J. Comment on "Interactions of manganese with the nitrogen cycle: Alternative pathways to dinitrogen" by G.W. Luther III, B. Sundby, B.L. Lewis, P.J. Brendel, and N. Silverberg .
Geochimica et Cosmochimica Acta 1998, 62 (12), 2217-2217.
Koopmans GF, Chardon WJ, Bril J, de Ruiter PC, Dolfing J. Applicability of immobilizing agents for the remediation of heavy metal polluted soils in the Netherlands. .
Wageningen: PGBO, 1998. Rapporten Programma Geïntegreerd Bodemonderzoek. 17 .
Römkens PFAM, Dolfing J. Effect of Ca on the solubility and molecular size distribution of DOC and Cu binding in soil solution samples .
Environmental Science & Technology 1998, 32 (3), 363-369.
Dolfing J. Halogenation of aromatic compounds: thermodynamic, mechanistic and ecological aspects .
FEMS Microbiology Letters 1998, 167 (2), 271-274.
Dolfing J. Degradation of monochlorinated and nonchlorinated aromatic compounds under iron-reducing conditions .
Applied and Environmental Microbiology 1996. , 62 (9), 3554-3554.
Mars AE, Houwing J, Dolfing J, Janssen DB. Degradation of toluene and trichloroethylene by Burkholderia cepacia G4 in growth-limited fed-batch culture .
Applied and Environmental Microbiology 1996, 62 (3), 886-891.
Dolfing J, Gottschal JC. Microbe-microbe interactions .
In: Mackie, R.I.; White, B.A.; Isaacson, R.E., ed. Gastrontestinal Microbiology: Volume 2, Gastrointestinal Microbes and Host Interactions . New York: Chapman & Hall, 1997, pp. 373-433.
Huang CL, Harrison BK, Madura J, Dolfing J. Gibbs free energies of formation of PCDDs: Evaluation of estimation methods and application for predicting dehalogenation pathways .
Environmental Toxicology and Chemistry 1996, 15 (6), 824-836.
Dolfing J, Prins RA. Methanogenic ''food chains'' .
ASM News 1996. , 62 (3), 117-118.
Dolfing J. Effects of electron donors and inhibitors on reductive dechlorination of 2,4,6-trichlorophenol - Comment .
Water Research 1995, 29 (7), 1811-1812.
Chen Q, Nijenhuis A, Preusting H, Dolfing J, Janssen DB, Witholt B. Effects of octane on the fatty acid composition and transition temperature of Pseudomonas-oleovorans membrane lipids during growth in two-liquid-phase continuous cultures .
Enzyme and Microbial Technology 1995, 17 (7), 647-652.
Dolfing J. Regiospecificity of chlorophenol reductive dechlorination by vitamin B12s .
Applied and Environmental Microbiology 1995. , 61 (6), 2450-2450.
Dolfing J, Beurskens JEM. The microbial logic and environmental significance of reductive dehalogenation .
Advances in Microbial Ecology 1995, 14 , 143-206.
Landa AS, Sipkema EM, Weijma J, Beenackers AA, Dolfing J, Janssen DB. Cometabolic degradation of trichloroethylene by Pseudomonas cepacia G4 in a chemostat with toluene as the primary substrate .
Applied and Environmental Microbiology 1994, 60 (9), 3368-3374.
Beurskens JEM, Dekker CGC, van den Heuvel H, Swart M, de Wolf J, Dolfing J. Dechlorination of chlorinated benzenes by an anerobic microbial consortium that selectively mediates the thermodynamic most favorable reactions .
Environmental Science & Technology 1994, 28 (4), 701-706.
Pries F, van der Ploeg JR, Dolfing J, Janssen DB. Degradation of halogenated aliphatic compounds: The role of adaptation .
FEMS Microbiology Reviews 1994, 15 (2-3), 279-295.
Dolfing J, Janssen DB. Estimates of Gibbs free energy of formation of chlorinated aliphatic compounds .
Biodegradation 1994, 5 (1), 21-28.
Holmes DA, Harrison BK, Dolfing J. Estimation of Gibbs free energies of formation for polychlorinated biphenyls .
Environmental Science & Technology 1993, 27 (4), 725-731.
Dolfing J, van den Wijngaard AJ, Janssen DB. Microbiological aspects of the removal of chlorinated hydrocarbons from air .
Biodegradation 1993, 4 (4), 261-282.
Dolfing J, Harrison BK. Redox and reduction potentials as parameters to predict the degradation pathway of chlorinated benzenes in anaerobic environments .
FEMS Microbiology Ecology 1993, 13 (1), 23-29.
Dolfing J, Harrison BK. Gibbs free energy of formation of halogenated aromatic compounds and their potential role as electron acceptors in anaerobic environments .
Environmental Science & Technology 1992, 26 (11), 2213-2218.
Dolfing J. The energetic consequences of hydrogen gradients in methanogenic ecosystems .
FEMS Microbiology Ecology 1992, 10 (3), 183-187.
Dolfing J, Tiedje JM. Acetate as a source of reducing equivalents in the reductive dechlorination of 2,5-dichlorobenzoate .
Archives of Microbiology 1991, 156 (5), 356-361.
Dolfing J, Tiedje JM. Influence of substituents on reductive dehalogenation of 3-chlorobenzoate analogs .
Applied and Environmental Microbiology 1991, 57 (3), 820-824.
Dolfing J, Tiedje JM. Kinetics of two complementary hydrogen sink reactions in a defined 3-chlorobenzoate degrading methanogenic co-culture .
FEMS Microbiology Ecology 1991, 86 (1), 25-32.
Dolfing J, Witholt B. Perspectives for the utilization of membrane-assisted sludge retention in municipal waste water treatment plants .
Lelystad: RIZA, 1991. Report RWZI-2000 91-03 .
Zeyer J, Eicher P, Dolfing J, Schwarzenbach RP. Anaerobic degradation of aromatic hydrocarbons .
In: International Workshop on Biotechnology and Biodegradation . 1989, Lisbon, Portugal: Gulf Publishing Company.
Dolfing J. Energetics and kinetics of two complementary hydrogen sink reactions in a defined 3-chlorobenzoate degrading methanogenic consortium .
In: Bélaich J-P; Bruschi M; Garcia J-L, ed. Microbiology and biochemistry of strict anaerobes involved in interspecies hydrogen transfer . New York: Plenum Press, 1990, pp. 151-160.
Dolfing J, Zeyer J, Binder-Eicher P, Schwarzenbach RP. Isolation and characterization of a bacterium that mineralizes toluene in the absence of molecular oxygen .
Archives of Microbiology 1990, 154 (4), 336-341.
Dolfing J. Reductive dechlorination of 3-chlorobenzoate is coupled to ATP production and growth in an anaerobic bacterium, strain DCB-1 .
Archives of Microbiology 1990, 153 (3), 264-266.
Dolfing J. Biorestoration .
Environmental Science & Technology 1989. , 23 (12), 1434-1434.
Dolfing J, Tiedje JM. Acetate inhibition of methanogenic, syntrophic benzoate degradation .
Applied and Environmental Microbiology 1988, 54 (7), 1871-1873.
Dolfing J. Acetogenesis .
In: Zehnder AJB, ed. Biology of Anaerobic Microorganisms . New York: John Wiley & Sons, 1988, pp. 417-468.
Dolfing J, Tiedje JM. Growth yield increase linked to reductive dechlorination in a defined 3-chlorobenzoate degrading methanogenic coculture .
Archives of Microbiology 1987, 149 (2), 102-105.
Dolfing J. Granulation in UASB Reactors .
Water Science and Technology 1986, 18 (12), 15-25.
Dolfing J, Tiedje JM. Hydrogen cycling in a three-tiered food web growing on the methanogenic conversion of 3-chlorobenzoate .
FEMS Microbiology Ecology 1986, 38 (5), 293-298.
Dolfing J, Bloemen WGBM. Activity measurements as a tool to characterize the microbial composition of methanogenic environments .
Journal of Microbiological Methods 1985, 4 (1), 1-12.
Dolfing J, Griffioen A, van Neerven ARW, Zevenhuizen LPTM. Chemical and bacteriological composition of granular methanogenic sludge .
Canadian Journal of Microbiology 1985, 31 (8), 744-750.
Dolfing J, Mulder J-W. Comparison of methane production rate and coenzyme F420 content of methanogenic consortia in anaerobic granular sludge .
Applied and Environmental Microbiology 1985, 49 (5), 1142-1145.
Dolfing J. Kinetics of methane formation by granular sludge at low substrate concentrations .
Applied Microbiology and Biotechnology 1985, 22 (1), 77-81.
ten Brummeler E, Hulshoff Pol LW, Dolfing J, Lettinga G, Zehnder AJB. Methanogenesis in an upflow anaerobic sludge blanket reactor at pH-6 on an acetate-propionate mixture .
Applied and Environmental Microbiology 1985, 49 (6), 1472-1477.
Hulshoff Pol LW, Dolfing J, van Straten K, de Zeeuw WJ, Lettinga G. Pelletization of anaerobic sludge in upflow sludge blanket reactors on sucrose containing substrates .
In: Third International Symposium on Microbial Ecology: Current Perspectives in Microbial Ecology . 1983, Michigan State University: American Society for Microbiology.
Koch M, Dolfing J, Wuhrmann K, Zehnder AJB. Pathways of propionate degradation by enriched methanogenic cultures .
Applied and Environmental Microbiology 1983, 45 (4), 1411-1414.
Hulshoff Pol L, Dolfing J, de Zeeuw W, Lettinga G. Cultivation of well adapted pelletized methanogenic sludge .
Biotechnology Letters 1982, 4 (5), 329-332.
Devault DA, Delmotte S, Macarie H, Dolfing J, Anschutz P. How early diagenesis reveals in situ biodegradation of herbicides in sediment .
In: Kortekamp, A, ed. Herbicides and Environment . Rijeca: InTech, 2011, pp.443-468.
Macarie H, Dolfing J. La Chlordecone (CLD) est-elle veritablement refractaire a une degradation microbienne ? .
In: Lessueur-Jannoyer, M, ed. Les Cahiers du PRAM . Le Lamentin, France: Pole de Recherche Agro-Environnementale de la Martinique, 2011, pp.25-30.
ZAhammad Sk, Yakubu A, Dolfing J, Graham DW. Feasibility tests for treating shampoo and hair colorant wastewaters using anaerobic processes .
Water Science and Technology 2011. In Press.